Our goal is to understand the mechanism and regulation of nuclear pre-mRNA splicing, an essential step in eukaryotic gene expression. Pre-mRNA splicing is catalyzed by a massive multimegadalton ribonucleoprotein machine called the spliceosome. Using the model organism S. cerevisiae as well as human cells, we apply a wide array of approaches, ranging from single molecule microscopy, biophysics, and chemical biology to biochemistry and cell biology to genetics and genomics to gain a deep understanding of how the spliceosome catalyzes and regulates pre-mRNA splicing, including in the context of transcription.
Jonathan Prescott Staley, PhD
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Chair of Molecular Genetics and Cell Biology
Professor of Molecular Genetics and Cell Biology
Committee on Genetics, Genomics and Systems Biology - Research and Scholarly Interests: ATPase, Baker's Yeast, Brewer's Yeast, cotranscriptional processes, DEAD-box RNA Helicases, DEAH-box RNA helicase, Exon, Gene Expression Regulation, genome-wide studies, Intron, mammalian proteins, mammalian systems, Messenger RNA, Nuclear Export, Pre-mRNA, RNA, RNA dependent ATPase, RNA Helicase, RNA Sequence, snRNA, Spliceosome, splicing, Yeast Proteins
- Websites: Staley Lab Website, Research Network Profile
- Contact: jstaley@uchicago.edu
- Graduate Programs: Cell & Molecular Biology, Genetics, Genomics & Systems Biology